Baculovirus Titer Assay

Proteos utilizes a robust economical plate-based flow cytometry assay for baculovirus titer determinations, allowing accurate optimization of infection parameters used for protein production in baculovirus infected insect cell cultures.

Understanding the multiplicity of infection (MOI) is an important step in the production of recombinant proteins using baculovirus infected insect cell expression platforms.  The MOI is a measure of the number of infectious virus particles used for infection relative to the number of insect cells in the culture.  Our experience producing hundreds of unique recombinant proteins has demonstrated that an accurate MOI is essential for predictable infection kinetics and reproducibility during multiple production runs.  An accurate titer is essential for calculating the MOI at the point of infection

The titer (infectious units (IU) per milliliter) of a baculovirus stock is determined by measuring the expression of gp64 (a baculovirus envelope protein) on the surface of infected insect cells eight hours post infection (hpi).  Our method measures the number of infected cells, in triplicate, over a series of nine dilutions of the baculovirus stock ranging from 1:2 to 1:100,000.  The assay is formatted for a standard 96 well plate and requires a minimum volume of baculovirus stock.

Unlike plaque and end-point dilution assays that are laborious and often add weeks to a project; our method is quick, reliable and economical.  Results can be obtained in as little as 3 business days from receipt of the samples, only requires 600 ┬ÁL of baculovirus stock, and is performed in triplicate so you can be confident of the results.  You will receive a full data sheet containing all of the assay data.

To order this service, please click here or on the order tab at the top of the page.

Questions regarding this service can be directed to meshuck@proteos.com or you can use the Contact Us form.

Baculovirus Titer Assay Figure 1