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Protein Expression

Expression platforms include mammalian cell, BV/insect cell, and bacterial systems. Molecular biology services include construct design, gene synthesis and endotoxin-free plasmid DNA isolation.

Recombinant Protein Expression Platforms

Our recombinant protein expression platforms are fully customizable, offering mammalian, insect, and bacterial systems with scalable transient production in shake flask culture in a wide range of scales up to 60 L. Each system offers distinct advantages depending on your protein’s complexity, desired yield, and downstream application. Not sure which expression platform to choose? Let our experts help design a customized workflow to meet your specific needs.  

 

Scalable Solutions and Quick Turnaround 

We deliver consistent, reproducible results with fast turnaround using optimized, validated protocols. Protein expression can typically be completed in 2–6 weeks as part of a streamlined 8–12 week gene-to-protein workflow. 

 

Mammalian Cell Expression 

Our industry-leading transient expression platform utilizes fully licensed HEK and CHO cell lines, providing rapid and reliable protein production. Mammalian expression systems are ideal for production of complex proteins, ensuring essential post-translational modifications that support proper folding, efficient secretion, and full biological activity. Whether you’re producing antibodies, enzymes, or other recombinant proteins, our platforms deliver the quality and performance you need for discovery and development. 

 

Insect Cell Expression 

Insect cell systems are well-suited for the production of both intracellular and secreted recombinant proteins, offering many of the post-translational modifications found in mammalian cells. Our baculovirus mediated expression platforms utilize fully licensed Sf9 (Spodoptera frugiperda) and Tni (Trichoplusia ni) insect cell lines to deliver high-yield, scalable protein production for a wide range of research applications. 

 

Bacterial (E. coli) Expression 

Our E. coli expression platform offers a rapid, cost-effective solution for producing high-quality recombinant proteins. It is ideally suited for proteins of prokaryotic origin or eukaryotic proteins that do not require complex post-translation modifications for proper folding and functionality. This platform is an excellent choice for expressing proteins of lower complexity, offering reliable performance and flexible scalability for a range of research applications. 

Frequently Asked Questions

Do you produce stable cell lines?

No, all proteins are produced in transient expression systems, allowing for production at scales ranging from milligrams to low gram quantities on an accelerated timeline. 

How do I determine which expression platform is right for my project?

Our team of experts can help you answer this question. Common factors to consider when selecting an expression platform include: 

  • Structural complexity of the protein 
  • Post-translational modifications 
  • Solubility 
  • Cellular localization 
  • Investment, balancing time versus cost 
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