Baculovirus-Infected Insect Cell (BIIC) Banking

Baculovirus-Infected Insect Cell (BIIC) Banking
11/28/2017

When you contract protein expression in baculovirus infected insect cells, Proteos retains your recombinant baculovirus in baculovirus-infected insect cell (BIIC) stocks for stable long-term storage.  The main advantage of this service is rapid scale-up and/or re-initiation of recombinant protein production in insect cell expression systems (Sf9 or Tni PRO).  Production of high titer baculovirus stock starting from a Baculovirus Expression Vector System (BEVS) compatible transfer vector is costly and time-consuming.  This upfront work is necessary for any initial production but BIIC banking allows you to skip this step on subsequent production runs; saving you time and money.  Utilizing this service, we can complete the expression portion of your project in weeks rather than months.

BIIC production begins by infecting a Sf9 insect cell culture at a multiplicity of infection (MOI) of 3 using recombinant baculovirus.  Culture parameters are monitored using a Beckman Coulter Vi-CELL TM XR and the cells are harvested 24 hours post infection.  An increase in average cell diameter of 10% or greater 24 hours post infection ensures that the insect cells were properly infected.  The BIIC stocks are cryopreserved and placed in the vapor phase of liquid nitrogen for long-term storage.  When you are ready for another production run, a BIIC stock will be thawed and used to infect a Sf9 cell culture for generation of high titer amplified virus stock.  The titer of the amplified virus stock is determined via our baculovirus titer assay [https://www.proteos.com/cro-services/assay-services/baculovirus-titer-assay] to ensure consistent results from one production run to the next.  BIIC stocks are stored at Proteos for 1 year following completion of the initial project (longer storage time is available by request).

Our BIIC banking service is always included in custom project quotations for baculovirus-mediated insect cell expression (click here to request a custom quote).  The BIIC banking service is also available for purchase on our website (click here to view our Order page) or as an add-on item for insect cell protein production packages (PP-003 and PP-007).  

Tags: BIIC banking, insect cell expression