Senior Scientist Cell Culture

Proteos is a wholly-owned subsidiary of NanoImaging Services, Inc (NIS). NIS and Proteos are global leaders in assisting pharma and biotech companies with discovery and development of therapeutics. We are currently seeking a highly motivated Cell Culture Senior Scientist to join the Protein Expression team, based in Kalamazoo, Michigan.

Functions and Responsibilities

• Maintenance and expansion of mammalian and insect cells in antibiotic-free shake flask culture
• Transient transfection of HEK and CHO cells
• Large scale endo-free plasmid DNA isolation
• Baculovirus production
• Baculovirus titer determination via flow cytometry assay
• Buffer and media preparation
• Protein analysis via SDS-PAGE and western blot
• Maintenance of cell and plasmid logs
• Sequence analysis
• Protein purification using affinity chromatography methods
• Maintain accurate records
• Accurately analyze and document data in technical documents
• Reports to Vice President Scientific Operations

Basic Qualifications – Education/Experience

• PhD with ≥3 years of relevant industry experience, or BS/MS with more than 7 years
• Comprehensive knowledge and hands-on experience in the areas of
  • Molecular biology
    • Sequence analysis
    • Primer selection
    • PCR
    • Plasmid DNA isolation
    • Aseptic technique
  • Cell biology
    • Biosafety level 2 (BSL-2)
    • Suspension culture of mammalian and insect cells
    • Transient transfection
    • Baculovirus mediated protein expression
    • Cell line development
  • Protein analysis
    • SDS-PAGE and western blot
  • Ability to work safely in a BSL-2 laboratory environment
  • Strong organization skills and ability to multi-task
  • Ability to work effectively in a fast-paced, team-oriented environment
  • Excellent written and verbal communication skills

Ideal qualifications

• Experience with large scale (60 L) antibiotic-free shake-flask cell culture
• Proficiency using the Bac-to-Bac system for baculovirus production
• Familiarity with the following instruments:
  • ViCELL
  • GelDoc GO gel imaging system
  • Thermocycler
  • Guava flow cytometer
  • Nanodrop
• Solid understanding of protein purification via affinity chromatography

Benefits

• Competitive salary
• 401K plan with company match
• Medical insurance
• Paid time off

Additional Requirements

• Successful applicants will be asked to show proof that they can legally work in the US, must pass a basic laboratory competency test, and provide an official transcript from highest completed degree
• Please submit cover letter and resume

In addition to the above the following are required for working in the Lab:

• Must be able to perform work for extended times in a biosafety cabinet.
• Must be available to work extended days and weekends as needed
• The person in this position needs mobility to move around lab and dock area
• The person in this position must be able to frequently exchange accurate information with peers, supervisor, and external clients.
• Frequently moves equipment/buckets that weight up to 50 pounds
• Constantly exposed to Biosafety Level 2 (BSL-2)

This job description is subject to change at any time.