Best Practices: Generating Multiple Expression Constructs

At Proteos, we pride ourselves on being a trusted partner in the world of recombinant protein production. Our commitment to delivering high-quality services that surpass client expectations is backed by decades of experience and a team of expert scientists. We anticipate and overcome challenges with innovative solutions tailored to your needs.

Unlocking Success in Recombinant Protein Production with Proteos

Navigating the complexities of recombinant protein production requires expertise and precision, especially when producing novel proteins. Generating multiple protein constructs through alteration of affinity tag selection, size, and placement can be highly beneficial for projects with no prior precedent of successful recombinant protein production.

Enhance Your Protein Production with Affinity Tags

Incorporating an affinity tag into your target protein simplifies purification, detection, and downstream assays. Affinity tags, which can also serve as fusion partners, often enhance the solubility and stability of recombinant proteins. However, the size, number, and placement of these tags can significantly influence the protein’s properties. That’s why creating multiple expression constructs and testing them in pilot experiments is crucial.

Optimizing Tag Configuration

The structural conformation of a protein can be altered by a large tag or multiple small tags. In such cases, using smaller or fewer tags might be a better option. The placement of the tag—whether at the N-terminus or C-terminus—can also affect the protein’s function and stability. Empirical testing during pilot experiments is essential to determine the optimal tag placement.

Protease Removal of Tags

Depending on your subsequent purification steps and downstream applications, you may need to remove the tags after protein expression. This process requires incorporating a protease recognition sequence during construct design. Testing tag removal, particularly for fusion partners, in pilot experiments ensures that the process does not render the protein insoluble.

Customized Solutions for Your Unique Needs

At Proteos, we tailor every project to your specific targets, needs, and end-use applications. Our milestone-driven workflows can include the generation of multiple constructs if necessary, allowing our highly skilled scientists to make data-driven decisions that mitigate risk and enhance the likelihood of successful outcomes.

Partner with Proteos for Your Protein Production Needs

Ready to take your recombinant protein production to the next level? Contact Proteos today and let our experts guide you through the complexities of protein production, ensuring your project’s success from start to finish. Together, we can achieve groundbreaking discoveries.