Fusion Protein Removal Following Mammalian Cell Expression

Proteos is an industry leader in the production of high-quality recombinant proteins for drug discovery research and will work closely with you to design mammalian cell expression constructs that are optimal for production of fit-for-purpose proteins.

Fusion proteins are often used to increase the expression and solubility of the target protein. However, a relatively large fusion partner may negatively affect the functionality of the target protein in biochemical or structural studies. Inclusion of a protease-specific cleavage site in the expression construct can facilitate removal of the fusion partner during purification.

This concludes our blog series, “Fusion proteins for mammalian cell expression”. This series has covered purpose, selection, placement, and removal of fusion partners from recombinant proteins produced in transient mammalian cell expression systems. If you missed the earlier information provided on this topic, follow the links below to read the previous blog posts in this series.

Part 1 – Fusion Proteins for Mammalian Cell Expression

Part 2 – Selecting a Fusion Protein for Mammalian Cell Expression

Part 3 – Fusion Protein Placement for Mammalian Cell Expression

Our team will work with you to design a customized workflow for transient protein expression in mammalian cells. Customized project designs that include multiple milestones are the cornerstone of data-driven decisions that lead to higher probabilities of project success. The Proteos team lets science lead the way. Contact us today to discuss your protein production needs.