Best Practices: Small-scale Expression Optimization

At Proteos, we take pride in being a reliable partner in recombinant protein production. With decades of experience and a team of expert scientists, we are committed to delivering exceptional services that exceed client expectations. We proactively address challenges and provide innovative, customized solutions to meet your unique needs.

The Value of Small-Scale Expression Optimization

Conducting small-scale pilot experiments is key to identifying the optimal expression conditions for recombinant proteins. These preliminary tests are invaluable, whether you’re using bacterial, insect, or mammalian expression systems. For instance:

• Bacterial Expression: In E. coli, testing different cell strains, growth mediums, and induction parameters such as temperature, duration, and inducer concentration can significantly enhance protein expression.
• Baculovirus-Infected Insect Cells: For insect cells, small-scale optimizations can identify the most suitable cell line, multiplicity of infection (MOI), and the best harvest time post-infection.
• Mammalian Cells: In mammalian cell expression, pilot experiments can pinpoint the ideal cell line, growth temperature, and harvest time, all while monitoring cell viability.

No matter which expression system is employed, investing time in optimizing conditions during pilot production can lead to drastically improved outcomes during large-scale production.

Your Comprehensive Guide to Recombinant Protein Production

This post concludes our blog series on best practices for novel recombinant protein production. For more detailed insights, check out our previous posts:

• Part 1 – Introduction
• Part 2 – Selecting an Expression System
• Part 3 – Generating Multiple Constructs

Partner with Proteos for Superior Protein Production

At Proteos, we understand that high-quality protein reagents are the foundation of successful drug discovery. Our team is ready to guide you through every step of the process, from expression optimization to large-scale production. Contact us today to discuss your project needs, and we’ll design a custom plan that aligns with your goals. Together, we’ll explore unique possibilities and overcome challenges to help you achieve success.